LOCUS       40924_5199        8936 bp DNA     circular SYN 17-DEC-2018
DEFINITION  PiggyBac transformation vector pB-UAS w+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     A toolkit for transformation and mutagenesis in Drosophila using 
            piggyBac
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Griswold CM, Roebuck J, Andersen RO, Stam LF, Spana EP.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2002) Invertebrate Targets, Syngenta 
            Biotechnology, Inc., 3054 Cornwallis Rd, Research Triangle Park, NC 
            27709, USA
REFERENCE   3  (bases 1 to 8936)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8936)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2002) Invertebrate Targets, Syngenta Biotechnology, Inc., 
            3054 Cornwallis Rd, Research Triangle Park, NC 27709, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  complement(11..620)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     protein_bind    646..740
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        759..997
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     1005..1021
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1202..1335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1372..1388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1396..1412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1420..1450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1465..1486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            1510..5629
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     mobile_element  complement(5629..6078)
                     /mobile_element_type="transposon:piggyBac 
                     transposableelement"
                     /label=ent
     promoter        complement(6096..6114)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6135..6151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6159..6175)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6183..6213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6228..6249)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6537..7125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7299..8156)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8157..8261)
                     /label=AmpR promoter
     rep_origin      complement(8287..8742)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8884..8900
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8910..8928
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctcttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
       61 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      121 tattaagttt tattatattt acacttacat actaataata aattcaacaa actatttatt
      181 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      241 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      301 tgttgtatta taaaataagt aattagctta acttatacat aatagaaaca aattatactt
      361 attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgac aaataacttt
      421 tttgcatttt ttgcacgatg catttgcctt tcgccttatt ttagaggggc agtaagtaca
      481 gtaagtacgt tttttcgtta ctggctcttc agtactgtca tctgatgtac caggcacttc
      541 atttggcaaa atattagaga tattatcgcg caaatatctc ttcaaagtag gagcttctaa
      601 acgcttacgc ataaacgatg acgtcccgcg ggcatgcctg caggtcggag tactgtcctc
      661 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      721 gcggagtact gtcctccgag cggagactct agcgagcgcc ggagtataaa tagaggcgct
      781 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
      841 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
      901 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
      961 ccaagaagta attattgaat acaagaagag aactctgcgg ccgctctaga actagtggat
     1021 cccccgggct gcaggaattc gatatcaagc ttgtccgagg gcaaaggaat agcacgtgct
     1081 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     1141 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1201 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1321 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1381 gtttcctgtg tgaaattgtt atccgctcac aattccacac accatacgag ccggaagcat
     1441 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1501 actctcgagc tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa
     1561 aaaacatttt ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat
     1621 ttacttcgat ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat
     1681 gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt
     1741 ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca
     1801 atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg
     1861 ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt
     1921 ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa
     1981 tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat
     2041 ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc
     2101 tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa
     2161 atcccaaacc gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt
     2221 tctcgctata taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca
     2281 ttttcgacta tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta
     2341 gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt
     2401 tgttcggggt tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca
     2461 gggtgacagc ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa
     2521 ctacggcacg ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact
     2581 agccgagaac ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc
     2641 gggctccgga tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca
     2701 cataccggcg cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg
     2761 atctgtgtga aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg
     2821 gcctatccgg gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg
     2881 ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga
     2941 ctgctcaatg gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag
     3001 caggatgacc tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg
     3061 gtgcggatgc cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc
     3121 caggagcttt cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa
     3181 ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg
     3241 ccgcttctga tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc
     3301 gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag
     3361 ccgtcttccg agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta
     3421 gctttcttgg gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt
     3481 tattaagggt atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg
     3541 tcctaccaac tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg
     3601 ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt
     3661 agcccgggat atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc
     3721 ggagaatggg tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg
     3781 atcctggctg tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac
     3841 ggtgagtggt tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg
     3901 ctagatttta gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt
     3961 atagtttata gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga
     4021 tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc
     4081 caacaactca cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc
     4141 aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt
     4201 gcatttcaat aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga
     4261 gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct
     4321 gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat
     4381 tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct
     4441 ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc
     4501 ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg
     4561 cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc
     4621 atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg
     4681 cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcccgga
     4741 gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat
     4801 cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta
     4861 gccgacatat atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt
     4921 tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga
     4981 tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt
     5041 tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc
     5101 tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc
     5161 ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc
     5221 tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg
     5281 attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa
     5341 atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat
     5401 atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg
     5461 attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat
     5521 ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat
     5581 aataagaata catttaattt agaaaatgct tggatttcac tggaactaga cgtcatcttc
     5641 acttacgtga tctgatattt cactgtcaga atcctcacca acaagctcgt catcgctttg
     5701 cagaagagca gagaggatat gctcatcgtc taaagaacta cccattttat tatatattag
     5761 tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
     5821 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
     5881 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
     5941 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaacgcaca gcgacggatt
     6001 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
     6061 tatctttcta gggttaaggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
     6121 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6181 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6241 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6301 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6361 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6421 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6481 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6541 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6601 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6661 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6721 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6781 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6841 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6901 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6961 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7021 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7081 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7141 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7201 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7261 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7321 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7381 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7441 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7501 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7561 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7621 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7681 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7741 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7801 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7861 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7921 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7981 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8041 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8101 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8161 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8221 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8281 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8341 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8401 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8521 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8701 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     8761 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     8821 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     8881 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattg
//
